제품/서비스

  • DiaPlexQ™ ZCD Detection Kit (ZIKV, CHIKV, DENV)_CE IVD.png
  • DiaPlexQ™ ZCD Detection Kit (ZIKV, CHIKV, DENV) ZIKA Virus, Chikungunya Virus, Dengue Virus의 감염여부를 정성 검출하는 검사시약
    Real-Time OneStep RT-PCR
    CE-IVD

    본 제품은 사람의 혈액, 혈장에서 추출한 RNA virus로 Real-Time OneStep RT-PCR 방식을 이용하여 ZIKA Virus, Chikungunya Virus, Dengue Virus 감염을 동시 또는 단독으로 검출하는 키트입니다.

제품사양
제품사양
DiaPlexQ™ ZCD Detection Kit (ZIKV, CHIKV, DENV)
Detection target Zika virus, Chikungunya virus and Dengue virus
Registration CE-IVD
Detection technology Real-Time OneStep RT-PCR
Specimen type 혈액(Blood), 혈장(plasma), 혈청(serum)
Analytical sensitivity 10 - 10² copies
Compatible instruments* ABI 7500 / 7500 Fast Real-Time PCR System (Applied Biosystems)
CFX96™ Real-Time PCR System (Bio-Rad)
PCR running time ~ 3 hrs
제품특징
제품특징

- Simple & Rapid detection system : OneStep Multiplex qRT-PCR based detection
- HotStart PCR system : High specific and sensitive result
- Reliable system : Automatic PCR control
- Positive control included
- CE certification

실험데이터
7-1.JPG
Sample type FAM JOE/VIC* CY5 Cal Fluor Red 610 Result
Positive Control + + + + Valid
Negative Control - - - + Valid
NTC (Non-Template Control) - - - + Valid
Sample case 1 + - - +/- ZIKV positive
Sample case 2 - + - +/- CHIKV positive
Sample case 3 - - + +/- DENV positive
Sample case 4 + + - +/- Positive (ZIKV, CHIKV)
Sample case 5 + - + +/- Positive (ZIKV, DENV)
Sample case 6 + + + +/- Positive (ZIKV, CHIKV, DENV)
Sample case 7 - - - - Required re-experiment
인용&논문
  1. First report of autochthonous transmission of Zika virus in Brazil. Zanluca C, de Melo VC, Mosimann AL, Dos Santos GI, Dos Santos CN, Luz K. Mem Inst Oswaldo Cruz.

    2015 Jun;110(4):569-72. doi: 10.1590/0074-02760150192. Epub 2015 Jun 9. PMID: 26061233

  2. Zika virus: following the path of dengue and chikungunya. Musso D, Cao-Lormeau VM, Gubler DJ.

    Lancet. 2015 Jul 18;386(9990):243-4. doi: 10.1016/S0140-6736(15)61273-9. No abstract available. PMID: 26194519

  3. Outbreak of Exanthematous Illness Associated with Zika, Chikungunya, and Dengue Viruses, Salvador, Brazil.

    Cardoso CW, Paploski IA, Kikuti M, Rodrigues MS, Silva MM, Campos GS, Sardi SI, Kitron U, Reis MG, Ribeiro GS. Emerg Infect Dis. 2015 Dec;21(12):2274-6. doi: 10.3201/eid2112.151167. No abstract available. PMID: 26584464

  4. Possible Association Between Zika Virus Infection and Microcephaly - Brazil, 2015.

    Schuler-Faccini L, Ribeiro EM, Feitosa IM, Horovitz DD, Cavalcanti DP, Pessoa A, Doriqui MJ, Neri JI, Neto JM, Wanderley HY, Cernach M, El-Husny AS, Pone MV, Serao CL, Sanseverino MT; Brazilian Medical Genetics Society–Zika Embryopathy Task Force. MMWR Morb Mortal Wkly Rep. 2016 Jan 29;65(3):59-62. doi: 10.15585/mmwr.mm6503e2.

주문정보 (Ordering information)
Technology Cat. No. Product Contents
Real-Time PCR SQD06-K020 (20 reaction) DiaPlexQ ™ ZCD Detection Kit (ZIKV, CHIKV, DENV) OneStep qRT-PCR Enzyme mix (ZCD)
2X OneStep qRT-PCR Buffer (ZCD)
Primer & Probe Mixture (ZCD)
Control Template (ZCD)
RNase free Water
SQD06-K100 (100 reaction)