제품/서비스

  • DiaPlexC™ Influenza Virus AB Detection Kit_CE IVD.png
  • DiaPlexC™ Influenza Virus A/B Detection Kit 인플루엔자바이러스 A/B의 단독감염 및 중복감염여부를 정성 검출하는 검사시약
    OneStep Multiplex RT-PCR
    CE-IVD

    본 제품은 Multiplex RT-PCR 방식을 이용하여 인플루엔자 바이러스 A와 B타입의 co-infection 및 single infection을 검사하는 키트로, 한 tube에서 RT (Reverse Transcription) 반응과 PCR 증폭반응이 순차적으로 진행됩니다. 별도의 추가 시약 필요 없이 키트 내에 포함되어 있는 시약만으로 PCR을 수행할 수 있으며, positive control인 Control Template가 제공됩니다.

제품사양
제품사양
DiaPlexC ™ Influenza Virus A/B Detection kit
Detection target Influenza Virus A , Influenza Virus B
Registration CE-IVD
Detection technology Conventional (End-point) OneStep Multiplex RT-PCR
Specimen type 비인두 swab(Nasopharyngeal swab), 인두 비강 흡입(Nasopharyngeal aspirate), 기관지폐포 세척액(Bronchoalveolar lavage (BAL)),
비강 swab(Nasal swab), 구강 인두 swab(Oropharyngeal swab), 객담(Sputum)
Analytical sensitivity 10 - 10² copies
Compatible instruments* ABI Veriti thermal Cycler (Applied Biosystems) recommended
PCR running time ~ 2 hrs 30 min
제품특징
제품특징

- Simple & Rapid detection system : OneStep Multiplex qRT-PCR (or RT-PCR) based detection
- HotStart PCR system : High specific and sensitive result
- Reliable system : Automatic PCR control & Internal control
- Positive control included
- CE certification

실험데이터
5-1.JPG
Sample type FAM Cy5 Texas Red / Cal Red 610 Result
Positive Control + + + Valid
Negative Contro - - + Valid
NTC (Non-Template Control) - - + Valid
Sample case 1 + - +/- Influenza A
Sample case 2 - + +/- Influenza B
Sample case 3 + + +/- Influenza A&B (Co-infection)
Sample case 4 - - + Negative
Sample case 5 - - - Required re-experiment

5-2.JPG
Lane Interpretation (detection)
1 Negative
2 Influenza Virus A
3 Influenza Virus B
4 NTC
5 Required re-experiment
인용&논문
  1. Fouchier RAV, Munster A, Wallenstein TM, Bestebroer S, Herfst D, Smith GF, et al.

    J Virol. 2002;79:2814-2822

  2. Lamb RA, Krug R. Field Virology, third ed.

    Lippincott-Raven Philadelphia. 1996; 1353-1395.

  3. Ha Y, Stevens, DJ, Wiley DC.

    The EMBO Journal. 2002;21;865-875.

  4. Russell RJ, Gamblin SJ, Haire LF, Stevens DJ, Xiao B, Ha Y, et al.

    Virology. 2004;287-296.

주문정보 (Ordering information)
Technology Cat. No. Product Contents
Conventional (End-point) PCR SMD42-K020 (20 reaction) DiaPlexC ™ Influenza Virus A/B Detection kit OneStep RT-PCR Enzyme mix (Inf A/B)
2X OneStep RT-PCR Buffer (Inf A/B)
Primer Mixture (Inf A/B)
Standard Marker (Inf A/B)
Control Template (Inf A/B)
RNase free Water
SMD42-K100 (100 reaction)