Product & Service

  • DiaPlexC™ G6PD Genotyping Kit (Asian type, African type)_CE-IVD.png
  • DiaPlexC™ G6PD Genotyping Kit (Asian type, African type) Multiplex allele-specific PCR based assay system for screen of G6PD deficiency
    CE-IVD

    G6PD, glucose-6-phosphate dehydrogenase is an enzyme found in the red blood cells (RBCs, erythrocytes). Its role is to protect RBCs from molecules called reactive oxygen that is usually formed in oxidative stress or after taking certain medication like antimalarial drugs. People with a G6PD deficiency can tolerate small amounts of these exposures, depending on the specific defect present in the gene. The G6PD deficiency is an X-linked recessive hereditary disease that is due to the single nucleotide polymorphism in certain target genes as mentioned below. In a G6PD deficiency condition, RBCs undergo a process called hemolysis that leads to anemia, even jaundice and kidney failure if untreated. A blood test may show as normal in a person with G6PD deficiency if it is done during or immediately following an acute episode of hemolysis. Genotyping of the G6PD gene is therefore important for taking early steps to maintain the health.

Specification
Specification
DiaPlexC ™ G6PD Genotyping Kit (African type) DiaPlexC ™ G6PD Genotyping Kit (Asian type)
Detection target 202 G → A
376 A → G
542 A → T
563 C → T (Mediterranean)
680 G → T
968 T → C
383 T → C (Vanua Lava)
487 G → A (Mahidol)
563 C → T (Mediterranean)
592 C → T (Coimbra)
871 G → A (Viangchan)
1360 C → T (Union)
1376 G → T (Canton)
1388 G → A (Kaiping)
Registration CE-IVD
Detection technology Conventional (End-point) Multiplex PCR
Specimen type Blood
Compatible instruments* ABI Veriti thermal Cycler (Applied Biosystems) recommended
PCR running time ~ 1 hr 30 min ~ 1 hr 30 min
Features
Features

- HotStart PCR system : Ultra high specific and sensitive result
- Multiplex PCR : Multiple targets in a single reaction
- Reliable system : Automatic Internal control
- Positive control included
- Easy-to-use master mix
- CE certification

Experimental
8-1.JPG
Lane Interpretation (detection) Lane Interpretation (detection)
1 Negative 5 542 A → T
2 376 A → G 6 680 G → T
3 202 G → A 7 968 T → C
4 563 C → T (Mediterranean) 8 Required re-experiment

8-2.JPG
Lane Interpretation (detection) Lane Interpretation (detection)
1 383 T → C (Vanua Lava) 6 1388 G → A (Kaiping)
2 592 C → T (Coimbra) 7 1376 G → T (Canton)
3 563 C → T (Mediterranean) 8 1360 C → T (Union)
4 487 G → A (Mahidol) 9 Negative
5 871 G → A (Viangchan) 10 Required re-experiment
Ordering information
Technology Cat. No. Product Contents
Conventional (End-point) PCR SHG11-K020
(20 reaction)
DiaPlexC™ G6PD Genotyping Kit (African type) 2X Multiplex PCR Smart mix (G6PD-African type)
Primer Mixture (G6PD-African type)
Standard Marker (G6PD-African type)
Mutant type Control (G6PD-African type)
Wild type Control (G6PD-African type)
Nuclease free Water
SHG11-K100
(100 reaction)
SHG16-K020
(20 reaction)
DiaPlexC™ G6PD Genotyping Kit (Asian type) 2X Multiplex PCR Smart mix (G6PD-Asian type)
Primer Mixture (G6PD-Asian type)
Standard Marker (G6PD-Asian type)
Mutant type Control (G6PD-Asian type)
Wild type Control (G6PD-Asian type)
Nuclease free Water
SHG16-K100
(100 reaction)